Abstract
The chloride channel blockers SITS (4-acetamido-4'-isothiocyanostibene-2,2'-disulphonic acid) and DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonic acid) markedly suppressed progesterone production in the Rana temporaria and Xenopus laevis follicle-enclosed oocytes and oocyte maturation stimulated by the homologous pituitary suspension and hCG, respectively. Inhibition was dose-dependent and decreased with the increase of the hormone concentration. SITS did not affect progesterone production in the R. temporaria follicle-enclosed oocytes stimulated by dbcAMP. Substitution of sodium chloride for equimolar concentrations of sodium gluconate, methanesulfonate, glutamate, or formate significantly potentiated the gonadotropin-stimulated progesterone production and oocyte maturation in the both species. Possible involvement of chloride channels in progesterone production by the gonadotropin-stimulated amphibian follicle-enclosed oocytes is discussed.
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