Involvement of catecholaminergic nerve fibers in angiotensin II-induced drinking in the Japanese quail, Coturnix coturnix japonica

Abstract

Monoamine distribution in a septohypothalamic area was investigated in the Japanese quail using a histochemical fluorescence method. This area includes the subfornical organ (SFO) and the preoptic area (POA) which are inferred dipsogenic receptor sites for angiotensin II (AII) in the Japanese quail. Nerve fibers showing yellow-green fluorescence were found between the POA and the SFO. They traversed from the POA to the SFO, and some fibers seemed to terminate on the neurons in the SFO. After a low dose of reserpine, a considerable number of fluorescent perikarya were found in the POA. These fibers and perikarya appeared to be of primary catecholamine judging from the fluorescence color. Following transection of these fibers, fluorescence disappeared from the fibers located on the SFO side of the transection plane, while it became a little more intense on the POA side. After transection, microinjection of AII into the POA was no longer effective in induction of drinking. On the other hand, sham operation or transection in areas other than between the POA and the SFO produced only minute changes in those fluorescent fibers and had little effect on the dipsogenic potency of AII injected into the POA. These results suggest that information of AII perceived at the POA is transferred to the SFO via those primary catecholamine-containing nerve fibers, which effect induced drinking.