INVOLVEMENT OF BDNF, NTRK2 AND PI3K IN THE MECHANISMS OF BINGE EATING AFTER PSYCHOGENIC STRESSORS IN ONTOGENESIS

Abstract

Aim. Study of binge eating after maternal deprivation or after rearing in social isolation on the expression of the Bdnf, Ntrk2 and Pi3k genes in the hypothalamus of rats. Methods. Animals from days 2 to 12 after birth were weaned from their mother for 10 days at 180 minutes; males aged 90-100 days were used in the experiments. Another group of animals was reared in individual cages from the 21st day after birth; males aged 90-100 days were used in the experiments. When developing binge eating, the animals received a high-carbohydrate diet (Nutella chocolate spread) for 1 hour every day or every third day within 30 days. 15 min before feeding, the paste was placed within 5 cm of reach with visual contact. Results. In groups with intermittent exposure to high-calorie food (the animals received pasta every third day), PCR analysis showed the presence of expression of the Bdnf, Ntrk2 and Pi3k genes in the hypothalamus. Expression of the Bdnf gene was higher in the group of rats after maternal deprivation compared to the control. It was also shown that the expression of the Ntrk2 and Pi3k genes on a high-carbohydrate diet was higher in rats reared in isolation compared to animals reared in the community. Conclusion. The data obtained suggest new pathways for the synthesis of pharmacological agents of a peptide nature associated with the PI3K/AKT/mTOR signaling pathway for the correction of food addiction caused by psychogenic stress in ontogenesis.