Abstract
Nanoparticles are considered viable options in the treatment of cancer. This study was conducted to investigate the effect of magnetite nanoparticles (MNPs) and magnetite folate core shell (MFCS) on leukemic and hepatocarcinoma cell cultures as well as their effect on the animal model of acute myelocytic leukemia (AML). Through current study nanoparticles were synthesized, characterized by various techniques, and their properties were studied to confirm their nanostructure. Invivo study, nanoparticles were evaluated to inspect their cytotoxic activity against SNU-182 (human hepatocellular carcinoma), K562 (human leukemia), and THLE2 (human normal epithelial liver) cells via MTT test. Apoptotic signaling proteins Bcl-2 and Caspase-3 expression were inspected through RT-PCR method. A cytotoxic effect of MNPs and MFCS was detected in previous cell cultures. Moreover, the apoptosis was identified through significant up-regulation of caspase-3, with Bcl-2 down-regulation. Invitro study, AML was induced in rats by N-methyl-N-nitrosourea followed by oral treatment with MNPS and MFCS. Biochemical indices such as aspartate and alanine amino transferases, and lactate dehydrogenase activities, uric acid, complete blood count, and Beta -2-microglubulin were assessed in serum. Immunophenotyping for CD34 and CD38 detection was performed. Liver, kidney, and bone marrow were microscopically examined. Bcl-2 promoter methylation, and mRNA levels were examined. Although, both MNPs and MFCS depict amelioration in biochemical parameters, MFCS alleviated them toward normal control. Anticancer activity of MNPs and MFCS was approved especially for AML. Whenever, administration of MFCS was more effective than MNPs. The present work is one of few studies used MFCS as anticancer agent.
Highlights
Cancer is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body (Kumari, 2020)
The current results revealed that folic acid-modified nanoparticles caused worse damage to cancer cells than to normal cells. folic acid coupled to the surface of iron oxide immobilized the surfaces of magnetic nanoparticles
Beta-2-microglobulin (B2M) is a subunit of the major histocompatibility complex (MHC) class I molecule; it is found on the surface of all nucleated cells and is especially abundant in lymphocytes and monocytes Under normal physiological conditions, some amount of Β2M may be secreted into circulation from or as a result of the intracellular release and is removed from blood predominantly by the kidneys
Summary
Cancer is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body (Kumari, 2020). Leukemia is blood cancer related to white blood cells. It arises when abnormal white blood cell begins to continuously replicate itself and not function normally. As they accumulate, they inhibit the production of other normal blood cells in the marrow, leading to anemia, bleeding, and recurrent infections. ALL is most common in children while AML mainly affects adults but can occur in children and adolescents (Joshi et al, 2013)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call