In-vitro Evaluation of Anti-oxidant and Anti-inflammatory Potential of Bio-synthesized Silver Nanoparticles from Endemic Medicinal Plant Species<i> Terminalia pallida</i>

Abstract

<i>Background:</i> The Seshachalam hill ranges of Eastern Ghats are known for their endemic flora. There were seven endemic species situated here in which present plant species <i>Terminalia pallida (T. pallida</i>) is one, this Endemic semi-tree species has a great medicinal value; at the same time, due to the presence of different types of secondary metabolites, they easily form nanoparticles and exhibit certain high-quality characteristics after being combined with noble metals like silver. <i>Objective:</i> These Silver Nanoparticles (SNPs) display peculiar characteristics in their nano level which shows more effectiveness compared to their raw extracts, with negligible side effects. The present work shows <i>in vitro</i> antioxidant (DPPH radical scavenging assay) and anti-inflammatory (inhibition of albumin denaturation) applications of SNPs synthesized from leaf, bark, and fruit extracts. <i>Method: </i>Antioxidant activity was carried out by Burits and Bucar method with slight changes and about anti-inflammatory (inhibition of albumin denaturation) assay was carried out by Dunnet method. <i>Result: </i>In these experiments, the results obtained were positive. The result, when compared among the three parts, showed more positive results in antioxidant assay SNPs synthesized from fruit, and dominated results in the anti-inflammatory test SNPs synthesized from the bark. <i>Conclusion: </i>By studying the applications of SNPs it is confirmed that the biosynthesized silver nanoparticles (SNPs) are helpful in the production of various medicines hence SNPs can be recommended ingredients for biomedicine.