In-vitro antiradical and inhibitory potential of Pentapetes phoenicea Linn. leaves against digestive enzymes related to diabetes

Abstract

BackgroundThe plant Pentapetes phoenicea has not been explored scientifically till now. The study was undertaken to establish the in-vitro antiradical property and α-amylase inhibitory activity to establish in-vitro hypoglycemic potential of various fractions of P. phoenicea leaves. MethodsThe 2,2-diphenyl-1-picrylhydrazyl (DPPH) test widely used to evaluate the antiradical activity of pure compounds or extracts was adopted to ascertain the antiradical property. The in-vitro α-amylase inhibitory assay using starch as a substrate and pancreatic amylase as the enzyme was used to ascertain the in-vitro hypoglycemic activity. Statistical and linear regression analysis was done using GraphPad prism 4.1 software. Results and discussionThe results for antiradical property showed promising IC50 22.93 & 36.30 μg/ml for aqueous and ethyl acetate fractions respectively in comparison with standard quercetin 6.089 μg/ml. The in-vitro-hypoglycemic activity of P. phoenicea at graded concentrations on α-amylase activity was observed with IC50 of 60.51, 74.01 μg/ml for ethyl acetate and aqueous fractions respectively as compared to standard drug acarbose with IC50 80.80 μg/ml. ConclusionFrom the results it was concluded that aqueous & ethyl acetate fractions showed promising IC50 values for in-vitro hypoglycemic activity. The preliminary investigation results confirmed the presence of flavonoids in ethyl acetate fraction, saponin, reducing sugar and tannin in aqueous fraction. Accordingly the antiradical activity and α-amylase activity may be regarded to the flavonoids, tannins and saponins present in the fractions.