Abstract
It is known that cells communicate through release of small molecules, but the precise communication mechanisms are not well understood. Through the use of single wall carbon nanotube sensor platforms, we can now quantify the gradients of nitric oxide that are release by each cell within an in vitro colony. To achieve this level of detail, a system was created to locate and identify cells, including differentiating between live and dead cells. The system then needed to identify changing fluorescence intensity values in relation to each cell and quantify these levels to allow for analysis and comparison. This new technology will allow researchers to understand how cells alter the release of active species during disease and healthy states, and potentially improve medical treatment through small molecule interactions.
Published Version
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