(Invited) New Advancements in Biosensor Technologies with Surface Plasmonic Resonance Microscopy

Abstract

Since the first use of surface plasmon resonance (SPR) technology for biosensing more than four decades ago, SPR has become a powerful tool for characterizing and quantifying biomolecular interactions, especially in the pharmaceutical industry for drug discovery. It has made great strides both in instrumentation developments and applications. One of the most significant developments is SPR microscopy, which integrates SPR technology with optical microscopy. SPR microscopy has enabled many new studies, such as direct measurements of small drug molecules interacting with GPCR proteins of a single cell in its native state, as well as direct measurement of drug responses on over hundreds of cells simultaneously. In this way, a heterogenous population of cells with different phenotypes, growth stages, and receptor expression levels may be directly studied to reveal deeper insight on receptor functionalities while in their native cellular state.