Abstract
Since the first use of the surface plasmon resonance (SPR) technology for biosensing more than two decades ago, SPR has become a powerful tool for characterizing and quantifying biomolecular interactions, especially in pharmaceutical industry for drug discovery. It has made great strides both in instrumentation developments and applications. One of the most significant developments is the SPR microscopy, which integrates the optical microscopy with SPR technology to enable many new studies such as direct measurements of small drug molecules interacting with GPCR proteins on cell membrane in their native state, and direct measurements of drug response over hundreds of cells with different phenotypes and growth stages to reveal their heterogeneity and other important functionalities. Here I will present some of the latest developments in SPR technology along with its applications in diverse fields.
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