Abstract
The conception of reagentless protein-based electrochemical biosensors that can detect multiple molecular analytes simultaneously directly in complex biofluids has the potential to transform our understanding of biology and personalized health monitoring. Previously, we have introduced an innovative protein-based electrochemical sensing strategy for the detection of glycans directly in unprocessed complex biofluids.[1] This innovative sensing technology uses redox reporter-engineered lectins as recognition elements. Lectins are glycan-binding proteins that can selectively interact with glycan molecules.[2] These engineered lectins are anchored to an electrode surface via a self-assembled monolayer of lubricin (LUB).[1] LUB is a glycoprotein with demonstrated antifouling properties, and it has proved its usefulness in sensors and biosensors as it allows electrochemical measurements to be performed directly in unprocessed biofluids.[2,3,4,5] In this sensing configuration, electrochemical signal generation (measured using voltammetric techniques) occurs when the target glycan binds to the lectin recognition element, diminishing the efficacy with which the attached redox reporter, methylene blue, transfers electrons to the electrode surface.[1] Thus, this is an on-off switch sensing mechanism. So far, our published work has only focused on the detection of single glycans such as Tn antigen. In this presentation, I will share our latest findings on how to provide a multiplexed capability to such a sensing technology and how it can be useful for in-situ monitoring of multiple protein biomarkers directly in biofluids, creating hence a new tool for biodiscovery and bioanalysis.
Published Version
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