Abstract
Abstract Cellulolytic fungus Gliocladium virens was found to exhibit very rapid growth and sensitivity to elevated temperature. Exposure to temperatures higher than 40°C caused irreversible inhibition of further growth. G. virens can metabolize an anti-metabolite, 2-deoxy-D-glucose (2dG) in preference to cellobiose. In combination with glucose or cellobiose, 2dG was consumed slower than glucose but sooner than cellobiose. Lignosulfonic acid and sorbitol did not influence germination nor growth, whereas dinitrophenol and oligomycin inhibited germination. Mutagenesis of G. virens using methylmethanesulfonate with UV-light yielded some mutants with 20–50% increase in filter-paper-hydrolyzing activity and an oligomycin resistant mutant, which formed ethanol when cultivated on glucose without aeration (2.7 g l −1 ethanol from 1% glucose).
Published Version
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