Abstract
Summary The influence of lactose and some other sugars on growth and β-galactosidase activity in callus tissues of Nemesia and Petunia was studied. In contrast to the other sugars, lactose showed a very pronounced inducing effect on β-galactosidase activity. The lactose induction was completely counteracted by actinomycin C1 and cycloheximide. Sucrose had a slightly stimulating effect on β-galactosidase activity in all its concentrations tested. In combination with lactose it increased the lactose induction of β-galactosidase activity, probably indirectly by improving the conditions within the calli. Galactose concentrations higher than 3% inhibited the development of β-galactosidase activity, and galactose combined with lactose removed the inducing effect of lactose. The mechanism of galactose action remains unclear. Studies on the kinetics of lactose induction and experiments, in which the calli were transferred from lactose to sucrose media, revealed that the lactose induction of fl-galactosidase activity was a long time process. The experimental data are consistent with an induction of β-galactosidase synthesis by one of its substrates, lactose.
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