Abstract
Shoot tips of newly emerging mango shoots were used as an explant and cultured on MS media for direct shooting. Different plant growth regulators i.e. BAP, NAA and IAA in varying concentrations were added to basal MS media. Experiment was laid out according to Completely Randomized Design (CRD) with four treatments and each treatment was replicated thrice. Maximum shoot induction (55.56%) was observed with 1 mg/L of BAP. Maximum mortality (83.30%) was observed when maximum concentration of BAP (3 mg/L) was used. Minimum days (12.633) to induce shoots were observed on MS media supplemented with 1 mg/L of BAP. Maximum number of shoots (2.50) were recorded in MS media supplemented with 1 mg/L of BAP. Low concentration of NAA initiated roots earlier in regenerated shoots as compare to high concentrations of NAA and IAA. MS media supplemented with NAA (1 mg/L) took less days (21 days) to induce roots. The combination of auxins (MS+ NAA 3 mg /L + IAA 1 mg /L) proved the best for root induction (38.87%). Keywords: Mangifera indica, tropical fruits, asexual propagation, plant tissue culture, micropropagation, phenolic exudation, acclimatization.
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