Investigations of Biological activities and Characterisation of Phytochemical constituents of Pulicaria laciniata extracts, an Endemic plant growing in south of Algeria

Abstract

Since limited research was conducted on studying the Pulicaria laciniata (P. laciniata (Coss and Kral)) aerial parts, this study was carried out. This study aimed to determine and classify three main extracts constituents by GC/MS, HPLC-PDA and screen there in vitro biological potency as the Antioxidant capacity, anti-diabetic, anti-Alzheimer's and anti-obesity activities. In which three extraction was affected with Chloroform, Ethyl acetate and n-Butanol each extract was characterised with GC/MS and HPLC-PDA, after that the capacities of the extracted molecules was evaluated against the ABTS free radical, ferric iron, α-amylase, α-glucosidase, acetylcholine esterase and the pancreatic lipase in order to investigate the mentioned activities. As a result, the n-Butanol and Ethyl acetate extracts had more polyphenols than the Chloroform extract. The most potent anti-obesity and anti- anti-alzeihmer action were demonstrated by the Chloroform extract through inhibition of pancreatic lipase and acetylcholinesterase.