INVESTIGATIONS CONCERNING THE VALUES OF SOME BIOCHEMICAL PLASMA COMPOUNDS IN WISTAR RATS GRAFTED IV. WITH A TRANSPLANTABLE TUMOUR (WALKER 256 ASCITIC CARCINOMA), CORRELATED WITH THE DAILY DEUTERIUM DEPLETED WATER INTAKE

Abstract

SUMMARY The aim of the experiment was to prove the clinica l and paraclinical (biochemical) effects of the daily deuterium depleted water intak e on the biological behaviour of the Walker 256 ascitic carcinoma grafted intravenous (iv) in t he Wistar rats, correlated to the values of some biochemical plasma compounds, like acid phosphatase (PAC), alaninaminotransferase (ALAT, GPT, ALT), aspartataminotransferase (ASAT, GOT, AST), gammaglutamiltranspeptidase (GGT), alchaline phosphatase (PAL). The experiment was conducted on 20 Wistar rats, which were distributed in 4 groups, each group containing 5 adult animals. First of all, the animals from group 2 and 4 were administrated ad libitum daily deuterium depleted water, for a period of six month s, and then inoculated (group 4) intravenously with Walker 256 ascite liquid (85 000 ‐ 90 000 tumour cells/ml ascite liquid) obtained from the Oncologic Institute “Testioreanu” , in Bucharest. During the first stage of the experiment (1 to 6 months) for all animals were weekly determined the body weight and some clinical aspects, regarding the food and water intake, some peculiarities of the behaviour and the health status. After intravenous inoculatio n of the Walker 256 ascitic carcinoma, all animals were rigurously observed, and were weekly evaluated from the point of view of the body weight evolution, respectively of some abnormal manifestations. Finaly, the experimental groups were sacrificed after 5 weeks f rom the intravenous inoculation with Walker 256 ascite carcinoma, under narcose with ether, by cardiac punction and blood samples collecting. The obtained results at the nec hropsic examination revealed the presence of the solid tumours at the level of some lymph nod e groups, lung and liver in 3 animals out of 5 in the case of the control tumour group (group 3). In comparance to this situation, in the case of group 4 (inoculated intravenously with Walk er 256 carcinoma and which consumed the deuterium depleted water), tumours weren’t obvi ously present in any of the animals. The blood samples were centrifuged and from the obtained plasma samples, the following enzymes were determined: acid phosphatase (PAC), alaninaminotransferase (ALAT, GPT, ALT), aspartataminotransferase (ASAT, GOT, AST), gammaglutamiltranspeptidase (GGT), alkaline phosphatase (PAL). The obtained results re vealed that the values of the determined enzymes are inside the normal limits for this speci es and animal category, respectively between