Investigation the effect of antibiotics on the content of N6-methyladenosine in rice seedling tissue and heavy metal on FTO activity based on antibody-free photoelectrochemcial biosensor

Abstract

Understanding the effects of environmental pollutants on RNA methylation and its relative enzymatic activity can provide new markers for the evaluation of the ecotoxicological effects of environmental pollutants. To achieve this aim, sensitive and selective detection technique for m6A and its relative enzyme is necessary. Herein, an antibody-free enzyme-assisted photoelectrochemical (PEC) biosensor for detection of m6A and fat mass and obesity-associated protein (FTO) was constructed based on enzymatic m6A structural change, where Se-MoSe2 nanoflower was selected as the photoactive material, gold nanoparticles (AuNPs) was used as recognition reagent, dm6AMP (N6-dithiolsitolmethyladenosine monophosphate, product of enzymatic m6A structural change and RNA hydrolysis) was used as the target molecule, and ZnSe was employed as signal amplification unit with the matched energy bandgap with Se-MoSe2 nanoflower. This biosensor can simultaneously identify m6AMP and FTO protein with high sensitivity and specificity. The effect of three kinds of antibiotics (Griseofulvin, sulfadiazine, lincomycin) on the content of m6A in the total RNA of the roots and leaves of rice seedlings were firstly investigated using PEC technique. Then, the effect of four heavy metals (AsO43-, Cd2+, Hg2+, Pb2+) on FTO protein activity was investigated with the IC50 value of 43.61, 55.58, 50.48 and 35.99 μg/L, respectively.