Abstract
Having made use of the micropipette aspiration technique, we here investigated the viscoelastic properties of hepatocellular carcinoma (HTC) cells from the view of cell cycle. The synchronous G 1 and S phase cells were achieved through thymine-2-desoryriboside and colchicine sequential blockage method and double thymine-2-desoryriboside blockage method, respectively. The synchronization results detected with flow cytometer showed that it could meet the requirements of the experiments nicely. Experimental results were analyzed with a standard linear solid viscoelastic model, in which an elastic element, K 1, is in parallel with a Maxwell element composed of another elastic element, K 2, in series with a viscous element, μ. The results indicated that high K 1, K 2 values and low μ value was the general characteristics of the cells; G 1 phase cells had higher K 1 values and low μ value than S phase cells, which endowed G 1 phase cells with higher elasticity and faster passive deformability than S phase cells. The results maybe also reflected the difference of cytoskeleton between G 1 and S phase cells, and suggested that G 1 phase cells were more suitable for surviving and metastasis in blood circulation than S phase cells.
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