Abstract
为了探索环糊精和寡肽的非共价相互作用,一定化学计量比的α-,β-,γ-环糊精（CD）分别和甘氨酸三肽（GGG）、甘氨酰-苯丙氨酰-苯丙氨酸三肽（GFF）在室温下反应达到平衡并用正离子模式质谱检测.实验结果显示GGG,GFF均可以和α-,β-,γ-CD生成1∶1配合比的非共价复合物.碰撞诱导解离实验进一步验证了α-,β-,γ-CD与GGG,GFF非共价复合物的形成.质谱滴定法测得的结合常数结果表明环糊精和两种三肽形成非共价复合物的结合强度均按照γ-,β-,α-CD的次序逐渐增大.GGG和α-,β-,γ-CD复合物的结合常数分别为2799.96,2528.73,1697.11 L mol-1,GFF和α-,β-,γ-CD复合物的结合常数分别为2773.94,2134.03,1330.68 L mol-1.对于α-,β-或γ-CD,含有苯基的GFF＋CD复合物的结合强度要小于相应的脂肪族的GGG＋CD复合物,表明虽然在气相GFF＋CD复合物的构象与溶液中的构象有所变化,但是苯基仍然参与和环糊精疏水腔体的键合作用.
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