Investigation of two mercury vapor collection techniques

Abstract

Objectives: The purpose of this investigation was to develop and test two in vitro mercury vapor collection techniques: a closed bottle technique (CB) and an intraoral flow (IOF) technique. Methods: Amalgam samples were prepared in acrylic first molars (#30) with standardized Class I preparations. In the CB technique, samples were placed in either a 25, 100 or 500 ml bottle (n=5). Vapor was analyzed with the Jerome M-411 using a syringe method over a 7 day period. In the IOF technique an impression of the lower right quadrant of a Typodont was taken with PVS impression material leaving a 5 mm space over #30. Samples were analyzed with the Jerome M-411 connected to the impression tray via tygon tubing at the buccal surface. Average mercury vapor release rates and standard deviations were calculated for each method. Data were analyzed by two-way ANOVA followed by Tukey HSD pairwise analysis for significant findings (α=0.05). Results: Both techniques indicated mercury vapor release was dependent on volume. The largest bottle, 500 ml, yielded a significantly greater (p≤0.00) amount of mercury vapor within the CB systems. In the IOF technique, the addition of air flow over the restoration demonstrated a significant increase (p≤0.05) in mercury vapor released compared to the sealed IOF technique. Significance: A method for mercury vapor analysis was developed for possible intraoral application. The IOF method with direct air flow removes possible saturation effects found in a CB system, while limiting external variables, which may contribute to errors associated with in vivo measurements.