Abstract
Tomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. In this study, 80 leaf samples were collected from tomato, pepper, cucumber and grapevine cultivation sites located in three different parts of Hakkari province: Şemdinli, Çukurca and Center districts. Real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) method was used for the detection of the virus. Amplification was carried out in reaction mix including QuantiNova Probe RT-PCR kit (Qiagen, Germany) using primers and TaqMan probe based on 3'-UTR (untranslated region) of virus, which amplified a 182 bp product of the genome. ToRSV was detected in 13 of the 80 samples and threshold cycle (CT) values ranged from 23.9 to 37.4. It was found that 16.25% of the samples collected from the districts of Hakkari province were found to be infected with ToRSV whereas no ToRSV was detected in the samples collected from the center of the city. The virus was detected on pepper and cucumber samples in Çukurca district, and it was also detected in tomato, pepper, cucumber and grapevine samples in Şemdinli district. To our knowledge, this study is the first report of molecular detection of ToRSV by real-time TaqMan RT-PCR in Turkey.
Highlights
Tomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae
The samples were collected in accordance with the common symptoms that are known to be caused by ToRSV on tomato, pepper, cucumber and grapevine (Figure 1)
The presence of ToRSV in Turkey has been reported in tomato, pepper, cucumber (Fidan 1995; Arlı-Sökmen & Şevik 2006), stone fruit (Azeri & Çiçek 1997), blackberry (Sertkaya 2010) and strawberry (Yeşilçöllü et al 2011)
Summary
Tomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. The most typical symptom of ToRSV infection in plants is the presence of annular spots on the leaves. It has other conspicuous symptoms in fruit trees and grapevines. The objective of this research is to determine the presence of ToRSV in tomato (Solanum lycopersicum), pepper (Capsicum annuum), cucumber (Cucumis sativus) and grapevine (Vitis vinifera) samples,collected from three different districts of Hakkari province, by real-time TaqMan RT-PCR method
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