Abstract
This study aimed to investigate time-dependent changes in spermatozoa DNA condensation and progressive motility and the correlation between DNA condensation and progressive motility of spermatozoa. Semen samples collected from 30 male patients were subdivided into two aliquots. The first aliquot was left unprocessed and labelled ‘raw’. The second aliquot was processed by a swim-up method and was evaluated at the 0th, 3rd and 24th hours. All samples were scored for routine semen analysis. Spermatozoa DNA condensation was evaluated by using the acridine orange and diff-quick stains. The percentages of the changes in the progressive motility and DNA condensation of spermatozoa were estimated. There were no significant differences between samples of the raw and the 0th, 3rd and 24th hours after processing in the percentage of spermatozoa DNA condensation. In all patients, the percentage of spermatozoa progressive motility was higher at the 0th and 3rd hours after processing than in the raw samples. There was no correlation between the percentages of the changes in spermatozoa DNA condensation and progressive motility. Spermatozoa selection can be performed reliably at the 3rd hour after processing of spermatozoa for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) applications. In addition, it is possible to say that the obtaining and processing of spermatozoa that can be performed on the day before the ovum pick-up (OPU) when it is required would not cause to any change in spermatozoa DNA condensation.
Highlights
Semen analysis is the first step in the evaluation of male infertility, including concentration, motility, progression, morphology and vitality evaluation of spermatozoa
The percentages of spermatozoa DNA condensation and progressive motility recorded at different times were summarized in the table
There were no significant differences between the samples of the raw and the 0th, 3rd, 24th hours after semen processing in the percentage of spermatozoa DNA condensation
Summary
Semen analysis is the first step in the evaluation of male infertility, including concentration, motility, progression, morphology and vitality evaluation of spermatozoa. Semen analysis could not provide exact information on fertility because there is a significant overlap between semen parameters of infertile and fertile men [1]. Evaluation of DNA integrity and maturation of spermatozoa has gained more importance in the diagnosis of male infertility. Significant differences in the levels of spermatozoa DNA damage among fertile and infertile men have been shown [2]. It is thought that spermatozoa DNA integrity provides more information compared to routine semen parameters for fertility evaluation [3]. Whether there is an association between the semen parameters and the DNA damage is not exactly known. While some studies have shown positive correlation between DNA damage of spermatozoa and abnormal semen parameters, others have found no correlation between DNA damage of spermatozoa and semen parameters
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