Abstract
Objective: The study aims to investigate the standardized, traceable dose amounts that will create the desired therapeutic physiological change in the tissue and prevent the risk of tissue damage in the light of metrology principles 
 Methods: In the study carried out in TÜBİTAK National Metrology Institute Medical Metrology Laboratory, a muscle phantom simulating the acoustic properties of muscle tissue was created and thermocouples were placed in it. Ultrasound at different intensities and durations at 1 MHz frequency was applied to the phantom. The temperature changes measured by the thermocouples were recorded. Each measurement was repeated three times and averaged 
 Results: 14 minutes application at 1 W/cm2 density, 10 minutes application at 1.5 W/cm2 density, ~7 minutes application at 2 W/cm2 density, and ~4 minutes application at 2.5 W/cm2 density have been achieved for the temperature range needed to produce therapeutic effect. 
 Conclusions: In order to achieve the therapeutic effect, the ultrasound doses used in the procedure should be checked. Measurements in multi-layer phantoms would be useful in future research.
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