Abstract
Originally chromatography was developed by M. Tswett as a preparative technique for the extraction of pure compounds from complex mixtures of natural origin. Development of preparative methods for isomer separation or product purification can be quite time consuming and an acceptable separation turns out to be impossible with the currently available technology. Preparative high-performance liquid chromatography (HPLC) combining high efficiency large diameter columns and short elution times widely extends the range of economical applications of the method. This chapter aims to achieve an empirical assessment of the advantages of using small particles in preparative HPLC by comparing the loading capacity observed for columns packed with similar materials but having different average particle sizes. The theory of preparative HPLC is most often approached as an extension of the theory of analytical HPLC. In preparative chromatography, self-displacement of the sample can be adjusted in certain cases and used to improve the performance of the column.
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