Investigation of The Effects of Zinc Oxide Nanoparticles Synthesized By Saccharomyces Cerevisiae Aqueous Lysate on In-Vitro Wound Healing Model

Abstract

Objective: The objective of this study was to examine the toxic and wound-healing behaviours of zinc oxide (ZnO) nanoparticles synthesized with an aqueous lysate of sourdough (Saccharomyces cerevisiae) on L929 mouse fibroblast cells.Method: Zinc oxide nanoparticles were synthesized by microwave method using the aqueous lysate of Saccharomyces cerevisiae. Characterization of ZnO nanoparticles was accomplished with Ultraviolet-Visible region spectroscopy (UV-Vis), SEM and Zeta sizer. The toxic behavior of ZnONPs at concentrations of 1, 10, 100, 1000 µg/mL and their effects on wound healing were investigated in-vitro in L929 cells.Results: A sharp peak was observed at 360-380 nm specific to ZnO in the UV spectrum. In the zeta analysis, the mean size of ZnO nanoparticles was 512.8±16 nm and the zeta charge was -30.38±3.12 mV. It was found that L929 cells treated with ZnONPs did not show dose-dependent manner. A significant increase was found in the wound closure amount of L929 cells applied 10, 100 and 1000 µg/mL ZnONPs compared to the control group cells.Conclusion: In-vitro wound healing effects of zinc oxide (ZnO) nanoparticles synthesized with Saccharomyces cerevisiae aqueous lysate show that these nanoparticles have the potential to be used in the pharmaceutical and cosmetic industries.