Investigation of the Effect of Propolis on Penicillin Induced Epileptiform Activity in Rats

Abstract

Aim: The aim of this study was to investigate the effects of propolis (PP), which has antioxidant and neuroprotective effects, on penicillin-induced epileptiform activity in rats. Material and Methods: Forty-two adult male Wistar rats were divided into 6 groups as control (CONT), penicillin (PEN), diazepam (DZM), only propolis (OPP), 50 mg/kg propolis (PP50), and 100 mg/kg propolis (PP100). ECoG recording was taken from rats. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels were determined from serum samples. Moreover, the latency of the first epileptiform activity, spike-wave frequency (SWF), and spike-wave amplitude (SWA) of the epileptiform activity were analyzed. Results: The latency of the DZM and PP100 groups was found to be longer than the CONT groups. The time-dependent SWF and total SWF of the PP50 and PP100 groups were lower than the CONT group. No significant difference was found between the groups in terms of SWA. SOD, CAT, and GPx levels were found to be higher, but the MDA level was lower in PP50 and PP100. Conclusion: As a result, propolis may be a potential antiepileptic drug candidate in the future with its antioxidant activity as well as prolonging latency and reducing SWF in epilepsy models.