Abstract
Тhe practical value of the culture liquid of probiotic bacteria was demonstrated. The culture fluid contains the products of vital activity of probiotic bacteria. It is the product of waste in the manufacture of classical probiotics. The culture liquid can be used to create metabiotics, due to the content of valuable exometabolites in its composition. The results of data on the positive effect of various amounts of culture liquid of propionic acid bacteria on the growth of bifidobacteria were presented. Microbiological studies have shown that 2 – 3 % of the filtrate was effectively reflected in the accumulation of biomass of bifidobacteria. The antagonistic activity of the culture fluid filtrate 2 – 3 % of the strain P.shermanii-4 against opportunistic (Escherichia coli-ATCC 25922, Staphylococcus aureus -ONU-223, Bacillus cereus-ATCC 11778) and pathogenic microorganisms (Salmonella enteritidis ‑ ONU-466) was studied. It was found that the use of a 2 % culture supernatant inhibits the growth of all opportunistic microorganisms, in addition to the pathogenic strain Salmonella enterica-ONU-466. With an increase in the dose of the filtrate, a small delay in the growth of Salmonella enteritidis-ONU-466 and an increase in the sensitivity of opportunistic microorganisms were observed.
Highlights
Various adverse effects of exogenous and endogenous nature on the human body cause changes in the immune response and can affect the qualitative and quantitative composition of the normal microbiota of the gastrointestinal tract (GIТ)
Probiotics based on living microorganisms, prebiotics
In our investigation found that a positive impact on increasing the biomass B. bifidum-1 was seen in all experimental samples after culturing at (37 ± 1) °C for 24 hours on a lactose medium with addition different amounts of supernatants culture fluid P. shermanii-4
Summary
Various adverse effects of exogenous and endogenous nature on the human body cause changes in the immune response and can affect the qualitative and quantitative composition of the normal microbiota of the gastrointestinal tract (GIТ). Probiotics based on living microorganisms (bifidobacteria, lactobacilli, propionic bacteria, etc.), prebiotics (indigestible substances stimulating the activity of certain microorganisms, symbiotics (combinations of probiotics and prebiotics), as well as metabolic probiotics – metabiotics (microbial metabolites) are presented in the arsenal of medicines aimed for the restoration of normal microbiota [1]. To determine the biocompatibility of probiotic preparations with autochthonous microflora, it is necessary to conduct a series of studies for a specific person or to develop autobiotics, which is a laborious process. In this regard, scientists have high hopes for a metabiosis that does not contain microorganisms, and lack the disadvantages mentioned above
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