Abstract
We studied tolerogenic properties of new nonpyrogenic low-endotoxic complex preparation composed of Shigella sonnei carbohydrate biopolymers under D-galactosamine and direct experimental endotoxic shock models. Pretreatment with complex preparation (exopolysaccharide + lipopolysaccharide) S. sonnei protects mice (CBA × C57B1/6)F1 against lipopolysaccharide (E. coli O:55)-mediated death. We demonstrate correlation between increasing preimmunization dose of complex preparation with rate of survival and reduction of TNF-α serum level under a direct endotoxic shock model.
Highlights
Ñåïñèñ, îáóñëîâëåííûé äåéñòâèåì ãðàìîòðèöàòåëüíûõ áàêòåðèé, ÿâëÿåòñÿ îäíîé èç ïðè÷èí ðàçâèòèÿ ñåðüåçíûõ îñëîæíåíèé ïðè õèðóðãè÷åñêèõ âìåøàòåëüñòâàõ, à òàêæå ïðè òðàâìàõ è îæîãàõ
Ñ èñïîëüçîâàíèåì ïðÿìîé ìîäåëè ýíäîòîêñè÷åñêîãî øîêà áûëî óñòàíîâëåíî, ÷òî ðàçâèòèå ñåïñèñà ïðîèñõîäèò â ðåçóëüòàòå îñòðîãî âîñïàëèòåëüíîãî ïðîöåññà â îòâåò íà áûñòðóþ ìàññèâíóþ ïðîäóêöèþ öèòîêèíîâ: ïðè ââåäåíèè ýíäîòîêñèíà ýêñïåðèìåíòàëüíûì æèâîòíûì èëè äîáðîâîëüöàì ó ïîñëåäíèõ îòìå÷àþò æàð, òàõèêàðäèþ è ñèñòåìíûå ñèìïòîìû âîñïàëåíèÿ, êîòîðûå ñîâïàäàþò ïî âðåìåíè ñ ïèêîì ÔÍÎ-a â ñûâîðîòêå [12], à âîçðàñòàíèå êîíöåíòðà
Ïîëó÷åííûå ðåçóëüòàòû ïîçâîëÿþò ñäåëàòü âûâîä, ÷òî ýôôåêò ïðè ïðîôèëàêòè÷åñêîì ââåäåíèè ïðåïàðàòà (ÝÏÑ + ËÏÑ) S. sonnei áûë îáåñïå÷åí ôåíîìåíîì ðàííåé ýíäîòîêñè÷åñêîé òîëåðàíòíîñòè
Summary
Îáóñëîâëåííûé äåéñòâèåì ãðàìîòðèöàòåëüíûõ áàêòåðèé, ÿâëÿåòñÿ îäíîé èç ïðè÷èí ðàçâèòèÿ ñåðüåçíûõ îñëîæíåíèé ïðè õèðóðãè÷åñêèõ âìåøàòåëüñòâàõ, à òàêæå ïðè òðàâìàõ è îæîãàõ.  íàñòîÿùåì èññëåäîâàíèè ìû ïðîâîäèëè îöåíêó ïðèíöèïèàëüíîé âîçìîæíîñòè êîððåêöèè ýíäîòîêñè÷åñêîãî øîêà ïðè ïðîôèëàêòè÷åñêîé èììóíèçàöèè ìûøåé (CBA 1⁄2 C57Bl/6)F1 íîâûì àïèðîãåííûì íèçêîýíäîòîêñè÷íûì êîìïëåêñíûì ïðåïàðàòîì íà îñíîâå ÁÓÏ S. sonnei — ýêçîïîëèñàõàðèäà (ÝÏÑ) è íàòèâíîãî (0,1% ïî ìàññå), îáëàäàþùåãî ïîëíîöåííîé ýíäîòîêñè÷åñêîé àêòèâíîñòüþ, ËÏÑ ñ èñïîëüçîâàíèåì ýêñïåðèìåíòàëüíûõ ìîäåëåé ýíäîòîêñè÷åñêîãî øîêà. Ìûøàì (CBA 1⁄2 C57B1/6)F1 â/á ââîäèëè ëåòàëüíóþ äîçó 2 ìã/ìûøü ËÏÑ E. coli Î:55 áåç ñåíñèáèëèçàòîðîâ ÷åðåç 72 ÷ ïîñëå èõ â/á èììóíèçàöèè êîìïëåêñíûì ïðåïàðàòîì (ÝÏÑ + ËÏÑ) S. sonnei â äîçàõ 100, 200 èëè 400 ìêã/ìûøü â 0,5 ìë ôèçðàñòâîðà.
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