Investigation of nonclinical toxicity of cosmetic nail gel in Caenrohabditis elegans model

Abstract

The study investigates the non-clinical toxicity of a cosmetic nail gel using the nematode model Caenorhabditis elegans. The research was motivated by the need for alternative methods to vertebrate animal testing due to ethical and legal considerations. The main objective was to evaluate the influence of cosmetic gel ingredients on the nematodes' behavioral and viability parameters. The N2 Bristol strain of C. elegans was used, exposed to different gel formulations. The methodology included analysis of egg hatching and L4 larval motility, comparing control and treated groups. The results showed no significant difference in egg hatching between groups, indicating that the gel components are not toxic to nematode reproduction. However, a decrease in the motility of L4 larvae exposed to the gel was observed, suggesting possible behavioral interference. The research concludes that while the formulation did not affect reproductive viability, the influence on motility requires further investigation to understand the nature of these behavioral changes. These findings underscore the relevance of using C. elegans as an alternative model for cosmetic safety evaluation.