Investigation of Microbial Elicitation of trans-Resveratrol and trans-Piceatannol in Peanut Callus Led to the Application of Chitin as a Potential Elicitor

Abstract

It is well-known that the invasion of microbes such as fungi in some plants, including peanut, can induce the biosynthesis of stilbenoids such as trans-resveratrol and trans-piceatannol. However, in a recent study it was found that not all kinds of microorganisms possessed such potential. The Gram-negative bacterium Pseudochrobactrum asaccharolyticum isolated from the peanut callus failed to act as an elicitor. After systematic investigation, the different inductive effects between fungi and Gram-negative bacteria were attributed to the chitin content of the cell wall. Results showed significantly more trans-resveratrol and trans-piceatannol was induced by fungi (8.92-16.35 and 2.15-7.01 microg/g of fresh calluses, respectively) than by bacteria (1.77-2.72 and 0.16-0.52 microg/g of fresh calluses, respectively), regardless of species and viability. Such great differences prompted the direct utilization of chitin, the distinctive component of fungal cell wall, as an elicitor. The results that trans-resveratrol induced by chitin was about two-thirds the amount induced by sterilized fungi, whereas trans-piceatannol (2.55+/-0.60 microg/g) was close to that by sterilized fungi, revealed chitin is not only an important fungal constituent responsible for the induction of trans-resveratrol and trans-piceatannol but also an efficient elicitor by itself. These findings suggested sterilized fungi and chitin can be used as a safe and fast elicitor, as far as the risk of viable microbes is concerned, to induce trans-resveratrol and trans-piceatannol in the well-controlled peanut tissue culture.