Abstract
Lysozyme is an enzyme from the group of hydrolases classified in the enzyme classification group EC 3.2.1.17. It was discovered by A. Fleming in 1922 as an element of the innate immune system and serves as a defense against Gram-positive and Gram-negative bacteria. It acts as a mucolytic and is present in many body fluids such as sweat, saliva and nasal secretions, as well as hen egg white, milk and expressed vegetables [1, 2]. It is known that latices of the plant family Euphorbiaceae Juss. show lysozyme activity [3]. In this study, latices of Euphorbia coerulescens Haw. and Euphorbia fortissima L.C. Leach were tested by MALDI-TOF-MS in terms of their sequence coverage with hen egg white lysozyme (HEWL). For this purpose relevant samples were separated by SDS-PAGE electrophoresis; the relevant protein bands were excised and processed using tryptic-in-gel-digestion. As a positive control, HEWL was treated like the latex samples. The results show that the 33 kDa bands in the samples of E. fortissima have a sequence coverage with HEWL of 36.7% and from E. coerulescens of 23,8%. Results with a sequence accordance greater than 20% and at least two major peptides detected are to be regarded as a significant positive [4]. By that, it can be concluded, that lysozyme is present in latices of Euphorbiaceae and seems to be a highly conserved protein comparable with isoenzymes in animals.
Published Version
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