Abstract
Using time-resolved imaging, we investigated the lysis of confluent PtK2 cell cultures by pulsed laser microbeam irradiation. Images obtained at time delays of 0.5 ns to 50 μs demonstrate lysis to be mediated by laser-induced plasma formation resulting in pressure wave propagation and cavitation bubble formation. Image analysis enabled quantitative characterization of the pressure wave and cavitation bubble dynamics. The zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansion as the primary agent of cell injury.
Highlights
Venugopalan and coworkers examined optical breakdownplasma formationin pure water produced by 6 ns laser pulses at ϭ532
nm when focused at high numerical apertureNA.[8]
They showed that the laser parameters used to produce cell lysis
Summary
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