Investigation of IL-6, IL-8 and TNF-α among patients infected with Proteus mirabilis in UTI Cases

Abstract

Aims: In human Urinary Tract Infection (UTI) consider one of the most common bacterial infections, the purpose of this research was to assess the association between Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Tumor Necrosis Factor-alpha (TNF-α) level in P. mirabilis UTI cases. Methodology: 160 samples of urine and blood were gotten from persons Suffer UTI, their age reached to (10-60) years, in addition to 100 samples of urine and blood from Healthy persons as a control group. All urine samples were inoculated by sterile loop immediately on blood agar plate and MacConkey agar plate and incubated aerobically at 37°C for 24 hr. Colony Forming Units (CFUs) method was used for growing single and pure bacterial colonies for target bacteria. Enzyme-Linked Immunosorbent Assay (ELISA) kit was used for detection level of human cytokines (IL-6, IL-8 and TNF-α) in inpatient and control groups. Results: The result showed that 62.5% of samples were positive for P. mirabilis. The level of IL-6, IL-8 and TNF-α considerably were high inpatient which reached to (312.189±0.916, 272.417±8.699 and 93.908±0.912)respectively, compared with control group (75.416±0.721, 77.978±0.932 and 51.777±0.556) respectively. There were important connotations between age, gender and placement and P. mirabilis infection. Conclusion: Target cytokines piece a critical role in UTI especially in acute phase. Also, there were substantial variances in age group, gender, and residency regarding infected with P. mirabilis and there are important relationships between IL-6, IL-8 and TNF-α in the patients infected with P. mirabilis.