Abstract
ABSTRACT In this article, the composition and distribution of bacteria associated with the gut of Tenebrio molitor (L.) larvae were investigated using both culture-dependent and culture-independent denaturing gradient gel electrophoresis (DGGE) methods. This work compares bacterial species associated with four different parts of T. molitor larvae gut: foregut, anterior midgut, posterior midgut, and hindgut. Five genera, Weissella, Lactococcus, Rahnella, Cronobacter, and Enterococcus, were isolated using nutrient agar. All of these strains were present in the posterior midgut and hindgut. The strains with milk-clotting activity in selective casein-plates assay were sequenced and identified as species of genera Weissella and Lactococcus, and those with proteolytic activity as Rahnella and Cronobacter, implying that they may be involved in protein utilization. But none of these strains showed cellulolytic activity. In DGGE experiment, 19 isolated bands belonging to nine taxa (Spiroplasma, Lactococcus, Lactob...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
