Abstract

The results revealed the activity of some digestive enzymes in the anterior, middle, posterior portions of midgut of third instar larvae of Eristalis megacephala and it showed that the highest pH of trypsin was at pH 6 in anterior mid-gut, middle mid-gut, and posterior mid-gut. The highest pH of chymotrypsin in anterior mid-gut was at pH 7, in the middle mid-gut it was nearly similar at all chosen pH values but slightly higher at pH 7 ,while the chymotrypsin activity in the posterior mid-gut of the larval stage of Eristalis megacephala was highest at pH 8.The highest activity of leucine – aminopeptidase was at pH 9 in anterior mid-gut, at pH 6 in middle mid-gut and at pH 7 in posterior mid-gut. The highest pH of carboxypeptidase A in the anterior mid-gut was at pH 6, in the middle mid-gut was at pH 7, while at pH 6 in the posterior mid-gut. The highest pH activity of carboxypeptidase B was 9 in anterior, middle and posterior mid-guts. Some glucosidases were studied and it showed that the highest pH of α- glucosidase was at pH 5.6 in anterior, middle and posterior mid-gut .The highest pH of β- glucosidase was at pH 6 in anterior, middle and posterior mid-gut. The highest pH of α- galactosidase was highest at pH 6 in anterior mid-gut and in the middle and posterior mid-gut at pH 5.6 .The highest pH of β- galactosidase was highest at pH 6 in anterior, middle and posterior mid-guts .The highest pH of α – amylase is at pH 3.6 in anterior mid-gut, pH 6 in middle mid-gut and pH 5.6 in posterior mid-gut. The present study also revealed that the activity of acid – phosphatase was high in anterior mid-gut that middle and posterior mid-gut while the activity of alkaline phosphatase was nearly similar in anterior, middle and posterior mid-gut but slightly higher in anterior mid-gut of larval stage of Eristalis megacephala.

Highlights

  • The Syrphidae make up one of the largest and most well defined Diptera families

  • The highest activity of chymotrypsin in anterior mid-gut was at pH 7 (Table 2), in the middle midgut it was nearly similar at all chosen pH values but slightly higher at pH 7 (Table 2), while the chymotrypsin activity in the posterior mid-gut of the larval stage of Eristalis megacephala was highest at pH 8 (Table 2)

  • Digestive proteases are the largest hydrolytic group of enzymes in insects that digest oligo- and di-peptideingested proteines (Terra and Ferriera, 2012). The enzymes in their active sites, their optima pH and their attacking positions on the protein molecule are categorized according to their composition

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Summary

Introduction

The Syrphidae (commonly referred to as hoverflies reflecting their flight modes) make up one of the largest and most well defined Diptera families. Adults especially composites, feed on nectar and pollen from the flowers, and are good pollinators. The larvae display various feeding practices: saprophagous, phytophagous and predaceous, and a few of them are scavengers in social insects' nests. Saprophagous larvae live in dung, wet decaying vegetation, wood, and tree sap. There are three larval instars, and the length of the life cycle varies greatly among species, taking less than two weeks in some, and possibly up to five years in others (Gilbert, 1993). The rat-tailed maggots (Syrphidae) prefer organically polluted sites and thrive in sewage and carrion

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