Investigation of Growth, Free Amino Acids, and Carbohydrate Concentration in the Roots of Perennial Ryegrass in Response to Soil Salinity at Subsurface Soil Depths

Abstract

Plants growing in salt-affected soils may have retarded growth and inhibited or altered metabolic processes. This study aims at investigating the impact of subsurface soil salinity on root growth and metabolic processes in perennial ryegrass (Lolium perenne). The seeds of perennial ryegrass (cv. Quick Start II) were planted in polyvinyl chloride (PVC) tubes (10 cm diameter × 42 cm long) for 2 months. The experiment consisted of three treatments: 1) control, 40 cm filled with sand–peat mixture (7 sand : 3 peat wt/wt); 2) T20, a 20-cm-deep layer of saline soil covered with a 20-cm-deep layer of sand–peat mixture; and 3) T30, a 30-cm-deep layer of saline soil covered with a 10-cm-deep layer of sand–peat mixture. Our study showed that soil salinity at the subsurface inhibited the growth of perennial ryegrass roots. Compared with the control, the root activity in saline soil layer decreased, whereas it remained high in the mixture-soil zone. The content of amino acids in the roots obtained from the surface soil (0–10 cm) in T30 was greater than that in both the T20 and the control regimes. The content of soluble sugars in the roots went up with the decrease of the depth of sand–peat mixture. The increased root activity and free amino acids content in the roots sampled from the upper soil layers coupled with the increased soluble sugars in the roots subjected to soil salinity stress in the bottom soil layer represents some adaptive responses and regulative mechanisms in perennial ryegrass.