Investigation of genotoxic potential of various sizes Fe2O3 nanoparticles with comet assay

Abstract

Event Abstract Back to Event Investigation of genotoxic potential of various sizes Fe2O3 nanoparticles with comet assay İbrahim H. Ciğerci1* and Şöhret Yüksek Kaygisiz1 1 Afyon Kocatepe University, Molecular Biology and Genetics, Türkiye Nanoparticles are found very common in nature. For instance, proteins exist in almost all biological systems and metal-oxide nanoparticles are easily produced etc. Iron oxide nanoparticles with unique magnetic properties have a high potential to use in several biomedical, bioengineering and in vivo applications, including tissue repair, magnetic resonance imaging, immunoassay, drug delivery, detoxification of biologic fluids, cell sorting, and hyperthermia. Although various surface modifications are being done for making these nonbiodegradable nanoparticles more biocompatible . But their toxic potential is still a major concern. Therefore, it is necessary to use further examination assay systems in order to check nanoparticle effects. In this study, genotoxic potential of <50 nm and <100 nm Fe2O3 nanoparticles were investigated by using Comet Assay. Allium cepa root meristems were exposed with five doses (0.001, 0.01, 0.1, 1, 10 mM) of <50 nm for 4 hour and three doses (2.5, 5 (EC50), 10 mM) for <100 nm of Fe2O3 nanoparticle for 24 and 96 h. Methyl methanesulfonate -MMS (10 ppm) was used as a positive control. The results were also analyzed statistically by using SPSS by Windows, 18.0. It was determined that different doses of <50 nm Fe2O3 nanoparticle have no genotoxic effect of DNA. Different doses of <100 nm Fe2O3 have no genotoxic but only 10 mM dose have genotoxic effect on DNA. When compared <50 nm with <100 nm of Fe2O3 nanoparticle; <50 nm have more effects than <100 nm of Fe2O3 on DNA damage. Keywords: Nanoparticles, Fe2O3, Comet Assay, Allium cepa, Genotoxic Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Poster Presentation Topic: Genotoxicity testing of chemicals and nanomaterials Citation: Ciğerci İH and Yüksek Kaygisiz Ş (2015). Investigation of genotoxic potential of various sizes Fe2O3 nanoparticles with comet assay. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00017 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Jun 2015; Published Online: 23 Jun 2015. * Correspondence: PhD. İbrahim H Ciğerci, Afyon Kocatepe University, Molecular Biology and Genetics, Afyonkarahisar, Türkiye, cigerci@aku.edu.tr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers İbrahim H Ciğerci Şöhret Yüksek Kaygisiz Google İbrahim H Ciğerci Şöhret Yüksek Kaygisiz Google Scholar İbrahim H Ciğerci Şöhret Yüksek Kaygisiz PubMed İbrahim H Ciğerci Şöhret Yüksek Kaygisiz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.