Investigation of Extraction and Characterization of Collagen from the Skin of Striped Catfish (Pangasianodon hypophthalmus)

Abstract

Collagen has been proved as a good biomaterial due to its high biological compatibility. For industrial purposes, collagen is extracted mainly from skins and bones of land animals. However, highly infectious and contagious diseases like BSE, TSE and FMD in pigs and cattle have limited the use of collagen derived from those animals. Fish skin, which is considered as an alternative source of collagen, provide the best raw material because of its high availability, no risk of disease transmission, no religious barriers and possibility of higher yielding collagen. In this project, collagen from the skin of striped catfish (Pangasianodon hypophthalmus) was extracted and characterized. SDS-PAGE resulted that our extracted collagen might be classified as type I collagen and consisted higher band intensity of β- and γ-chains. Fourier transform infrared (FT-IR) spectra showed that the extracted collagen consist main figurative peaks of amide bands similar to collagen from other source such as rattail tendon and earthworm cuticle. Results showed that the modified process of extraction collagen from striped catfish skin was investigated and the extracted collagen is suitable for artificial applications in tissue engineering.