Abstract
Collagen is an extracellular matrix protein with great importance in biomedical application. The search for collagen from various sources is intensified especially from marine source. This study was carried out to extract the collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential in vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE. MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929 skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain. At 100 µg/cm2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of fibroblasts cell and has potential to be used as an alternative source of collagen.
Highlights
Clarias batrachus, known as ikan keli in Malay is a native species found predominantly in river of south-east Asia
Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain
At 100 μg/cm2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05)
Summary
Known as ikan keli in Malay is a native species found predominantly in river of south-east Asia. C. batrachus is a type of fresh water fish inhabiting fresh water, brackish water, muddy marshes, as well as stagnant water (Emenike et al, 2012; Saha et al, 2011). Efforts were spent to develop collagen as scaffold to aid in wound healing, facilitate cytokine transportation to reduce inflammation and promote proliferation stage, in hope to speed up healing process (Enoch & Leaper 2008; Hart et al, 2002; Ravari et al, 2011; Varga et al, 2014; Williamson & Harding 2004; Yan et al, 2010). With the advancement of this study, we are seeking to develop a cheaper and readily available fish collagen from local source
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