Abstract
Copolymers of styrene and ethyl methacrylate have been separated according to composition by gradient HPLC on silica columns or CN bonded phase columns. This mode of separation according to composition was applied to fractions obtained by size exclusion chromatography (SEC). From viscosity and molecular mass data of copolymers with a styrene content ranging from 7.5 to 95.3 mass-% it can be concluded that SEC separates mainly by molecular mass even in this copolymer system. Thus, chromatographic cross-fractionation is possible by prefractionation by SEC and subsequent separation according to composition by gradient HPLC.
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