Abstract
Cold atmospheric plasma is considered to be an effective wound-treatment technique because of its efficient bactericidal properties and gentleness to the human body. The purpose of this study was to explore a new way of generating plasma that produces large amounts of N2O5, which exhibits superior biological effects compared to O3. This method is referred to as “combination mode” because it combines both dielectric barrier discharge and gliding arc discharge. A plasma-generating device was designed according to the principles of this combination mode. This outputs plasma-activated air (PAA) for both convenience and effectiveness. We sought to verify the effectiveness of PAA in the treatment of naturally infected wounds. The results of in vivo experiments showed that all groups treated with PAA had better wound-healing profiles than the control group, and the group treated with combination-mode plasma for 3 min had the best performance. The results of 16S rDNA analysis demonstrated that PAA is a good inhibitor of the most common infection-causing bacteria. Immunohistochemistry analysis revealed an increase in the secretion of growth factors and a decrease in both inflammatory and anti-inflammatory factors in the wound tissue of the treated groups. Finally, preliminary indications of the biosafety of PAA were provided by considering the body-weight trends in the animals and examining tissue sections from their five major organs. This study confirms the positive effect of combination-mode plasma on the healing of infected wounds and explores its mechanisms from multiple dimensions, contributing to the further application of PAA in clinical treatment.
Published Version
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