Abstract
AbstractThe effectiveness of biobarrier for leachate containment was investigated through continuous flow column studies. Landfill leachate collected from Ariyamangalam open dumping site was used as the source for microorganism isolation. Isolated organisms were enriched in the presence of lead to selectively obtain lead-tolerant microorganisms. The biochemical analysis results confirmed that the isolated strain is gram positive and rod shaped, Bacillus insolitus. Synthetic leachate was inoculated with different cultures of bacteria and was aerated to an initial dissolved oxygen level of 7.8 mg/L. In the batch reactor, chemical oxygen demand (COD) and lead removal was higher for lead-enriched cultures (62 and 28%) when compared with the nonenriched cultures (57 and 13%). The initial hydraulic conductivity of 1.99×10−2 cm/s was reduced to 1.1×10−3 cm/s at the end of 36 days of continuous study of the microbial sand column (MC), whereas negligible reduction was observed in blank column without microbes (...
Published Version
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