Abstract
Aim: To evaluate the in vitro antioxidant potential and total phenolic contents of the methanolic extract of Swertia chirata . Place and Duration: Department of Botany, Dr. H. S. Gour University (HSGVV), Sagar, between February 2010 to July 2010. Methodology : The plant material (aerial part) was subjected to defatting with petroleum ether then successively extracted with methanol. Total phenolic contents of methanolic extract was determined using the Folin-Ciocalteau reagent method while in vitro antioxidant potential was evaluated by using DPPH, hydroxyl radical, nitric oxide radical scavenging as well as ferric reducing power assays. Results: The total phenolic content in 1 mg of methanolic extract of S. chirata was equivalent to 4.5 µg Catechol. The IC 50 value of the DPPH method, hydroxyl radical and nitric oxide radical scavenging activity was 222.74±0.19, 307.93±0.10 and 870.55±0.20
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