Investigation of antioxidant activity and antiurease, anticollagenase enzyme inhibition profile of oleaster seeds

Abstract

In this study, the inhibitory effects of extracts prepared from oleaster seeds on antioxidant activity, anti-urease and anti-collagenase enzyme activities, which have an important area in health, were investigated. For this purpose, the inhibitory effects of aqueous and methanolic extracts of these oleaster seeds on these enzymes were analyzed by spectrophotometric measurements. The inhibitory effect of the prepared extracts on these enzymes was evaluated by in vitro method. Only the aqueous extract was found to show both anti-urease and anti-collagenase activity. It was determined that it showed higher inhibition activity than the urease standard. Accordingly, the anti-urease IC50 value of the extract was 0.23 ± 0.003μg/mL; the % inhibition value at 2.8 µg/mL extract concentration was determined as 36.54 ± 1.11. Anti-collagenase IC50 value 2.8±1.12 μg/mL; the % inhibition value at 4.6 µg/mL extract concentration was determined as 50.7±0.01. The IC50 value of epigallocatechingallate anti-collagenase, which is the anti-collagenase standard, was determined as 1.05±0.002 μg/mL. DPPH and ABTS methods were used to examine the antioxidant activities of oleaster seeds extracts. According to DPPH results, while aqueous extract provided 25,85 % removal, the methanolic extract provided 20,44 % removal. In ABTS removal activity, while aqueous extract provided 25,55 % removal, the methanolic extract provided 18,23% removal. This study clearly shows that oleaster seeds the aqueous extract of can be used as an alternative source of anti-urease and anti-collagenase source. Due to the important role of these enzymes in various diseases, new drugs with inducing or inhibitory effects should be developed.