Abstract
BackgroundBrucellosis is a worldwide zoonotic disease that causes serious public health problems. This study aimed to identify Brucella strains isolated from various clinical samples by conventional and molecular methods and to determine antimicrobial susceptibilities against doxycycline (DOX), streptomycin (STR), ciprofloxacin (CIP) and rifampicin (RIF) by the gradient strip (E test) test method.MethodsA total of 87 Brucella strains isolated from various clinical specimens between 2004 and 2018 were included in this study. While four of the 87 strains included in the study were identified only at the genus level, the remaining 83 strains were identified at the species level by the Real-Time Multiplex PCR (M-RT-PCR) method and conventional methods were used for biotyping.ResultsAccording to molecular identification results, 83 strains were identified as B. melitensis by the M-RT-PCR method, with 82 strains identified as Brucella melitensis biovar (bv) 3 and one as B. melitensis bv 1 according to the conventional biotyping method. Among the antibiotics studied, CIP was found to be the most active agent according to the minimum inhibitory concentrations (MIC)90 values. This was followed by DOX and STR, respectively. While all of the isolates were sensitive to CIP, DOX and STR, 18 (20.7%) strains were found to be moderately susceptible to RIF, with the highest values of MIC50 and MIC90.ConclusionsIn our study, all strains were identified as B. melitensis. DOX, STR, CIP and RIF used in the treatment of brucellosis were found to be effective.
Highlights
Brucellosis is a worldwide zoonotic disease that causes serious public health problems
The isolation, identification and biotyping of the Brucella species are very important for both epidemiological studies and eradication programs
Information about the species and biotype distribution of Brucella is important as it will contribute to the follow-up of biotypes and vaccine strains in Turkey as well as the selection of optimal strains used in serological diagnosis [6]
Summary
Brucellosis is a worldwide zoonotic disease that causes serious public health problems. The Brucella species is a zoonotic infectious agent that can be transmitted to humans by direct contact with body secretions by impaired skin, inhalation and conjunctiva, as well as the consumption of meat, milk and milk products of animals, such as infected sheep, goats, cattle and pigs. With the appropriate clinical manifestations, the diagnosis of the disease occurs by serological tests and the isolation of the agent [1, 3]. Definitive diagnosis is isolation of bacteria from culture in brucellosis, serological methods are mostly preferred, due to the difficulties in isolating the agent most of the time, especially in chronic cases, the risk of laboratory infection, and the delayed results [4]. The isolation, identification and biotyping of the Brucella species are very important for both epidemiological studies and eradication programs. Information about the species and biotype distribution of Brucella is important as it will contribute to the follow-up of biotypes and vaccine strains in Turkey as well as the selection of optimal strains used in serological diagnosis [6]
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