Investigation of Antibiotic Resistance Brucella spp. Isolated from Aborted Animal Fetuses

Abstract

Antibiotic resistance is a global threat to human and animal health especially when it related with a zoonotic disease like brucellosis which treated with the same bunch of antibiotic for long decades. This issue raised a question of whether Brucella spp. have gain resistance to the commonly used antibiotics. To study Brucella spp. antibiotic susceptibility, samples from (20) aborted fetuses (12 sheep and 8 buffalo) were used. It submitted to PCR and culture methods for detection and microbial isolation and (7) kinds of antibiotic for the susceptibility test. Fifteen Brucella culture was isolated (9) from sheep fetuses and (6) from buffalo. The biotyping of the isolates revealed that they were Br. melitensis and Br. abortus respectively. The overall agreement between PCR and culture reached (95%) with the superiority of PCR over microbiological culture. Susceptibility tests indicate that (tetracycline, ciprofloxacin and gentamicin) still efficient while rifampicin and cephalexin show moderate results at last comes neomycin and erythromycin which were not efficient. In conclusion, Brucella spp. did not gain resistance trait to the main antibiotic used in it treatment.