Abstract
Summary The establishment and maintenance of suspension cultures Cinchona ledgeriana Moens have proved difficult but cultures have been established in Gamborg's B5 medium supplemented with 10 % coconut milk or 1 % polyvinylpyrrolidone (PVP). The loss of viability in cultures without these supplements is associated with a sustained drop in pH. Both coconut milk and PVP increase the resistance of B5 medium to changes in pH which may explain their success in maintaining viability in cultures of Cinchona . PVP does, however, have its limitations as it appears to bind both quinine and quinidine which prevents the detection of products in Cinchona cultures.
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