Abstract
Background: Hypertension (HTN) is a major risk factor for Cerebrovascular and cardiovas-cular diseases, causing premature deaths. Hence, there is an urgent need for predictive bi-omarkers to detect HTN before its onset. Methods • Cell Culture Technique: Growth of HFAs, followed by conversion of normal (A2) HFAs intoreactive (A1) type, by using ATP solution. • Confocal Microscopy: Quantification of Glial Fibrillary Acidic Protein (GFAP) in A1 and A2 HFAs, by using antibody ab 7260 with AF 488. Proteomics Methos • Extraction of proteins from A1 and A2 HFAs, using Single-pot, Sol-id-phase-enhanced Sample Preparation (SP3) protocol. • Liquid Chromatography- Mass Spectrometry techniques for obtaining the lev-els of calcium-gated proteins in A1 and A2 cell-solutions. Results • Micrographs obtained by Leica Microsystem indicated thicker filaments in A1 HFAs compared to A2 HFAs. • Confocal results showed higher levels of GFAP in A1 HFAs (P> 0.05). • Proteomics data analysed by PEAKS software revealed that proteins, such as GFAP were higher in reactive HFAs. Conclusions: The results showed higher levels of GFAP in A1 HFAs. Therefore, early detection of GFAP in human body fluids could be a reliable predictive biomarker for HTN
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