Abstract
The goal of this work is to differentiate canine bone marrow mesenchymal stem cells (BMSCs) into pancreas islet-like cells in vitro. Healthy dogs' BMSCs were isolated and cultivated in the laboratory. Two-step induction procedures were used to cause BMSCs to develop into islet-like cells after the third passage (at 80% confluence). Cell morphology, expression of the canine insulin gene by reverse transcriptase PCR (RT-PCR) and quantitative PCR (qPCR), detection of nestin and insulin proteins by immunocytochemistry, and quantification of insulin level in culture media were all used to characterise differentiated BMSC cells into islet-like cells. Under the bright field microscope, typical grape-like cell clusters could be seen.Insulin gene and protein expression were discovered in differentiated cells. Insulin levels in differentiated cells' culture media were abnormally high. Canine BMSCs were found to be capable of being differentiated into islet-like cells. These differentiated cells may have played a key role in canine diabetes therapy. Key words: Dogs, BMSCs, Islet-like cells, Differentiation
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