Abstract
Abstract Dendritic cells (DC) and monocytes, which link the innate and adaptive immune response, can be stimulated with lipopolysaccharide (LPS) via toll-like receptor 4 recognition. However, understanding of human cell-specific responses to different doses of stimuli (LPS) is limited. We investigated the monocyte and DC specific, as well as the overall inflammatory response following exposure to varying doses of LPS. Fresh peripheral whole blood (n=8) was used in an in-vitro whole blood culture model to assess DC and monocyte responses in co-culture with varying doses of LPS (0.25 μg/mL, 0.5 μg/mL, 0.75 μg/mL, 1 μg/mL). DC and monocyte cytokine responses were measured via flow cytometry. Supernatants collected from the in-vitro model were used to assess the overall inflammatory response using a cytometric bead array. Exposure to all doses of LPS tested increased monocyte and DC specific cytokine production, and the overall leucocyte response. DC and monocyte cytokine production decreased in a dose-dependent manner when exposed to higher LPS doses. Our data suggests LPS concentration impacts on cell-subset specific responses more than the overall inflammatory response. Assays that assess the overall inflammatory response may lack the sensitivity required to elucidate underlying pathophysiology of infection and inflammation.
Published Version
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