Abstract
Objective To investigate the possibilities and terms of endothelial progenitor cells (EPCs) derived from mouse bone marrow forming the tube and invading the hepatocellular carcinoma cells H22 Clusters. Methods Bone marrow mononuclear cells were gained by density-gradient centrifugation.After culture for 1 h and 24 h, the nonadherent cells were extracted with the method of sequence of differential adhesion and then were induced by EGM-2 medium. Cell multiplication was tested by methyl thiazol tetrazolium (MTT) chromatometry at 1, 3, 7, 10, 13, 16, 20 days. The cells were identified by cell morphology, immunofluorescence and flow cytometry analysis, and the abilities of forming the tube and invading the hepatocellular carcinoma cells were observed after culture for 1 week. Results The EPCs grew as colony-forming units within 1 week and as cobblestones after 1 week. The cells could phagocytize the lowdensity lipoprotein and membrane binded Ulex Europaeus agglutinin with double-positive percentage (96. 46 ± 1.74)%. The positive expression rate of CD34 and vascular endothelial growth factor receptor (VEGFR)-2 was (91.23 ±3.76)% and (92. 85 ±2. 12)% respectively, and that of CD133 was (61.54 ± 13.71)%. EPCs could form tubes and recruit to the tumor cells. Conclusion EPCs were rich in mouse marrow and could be obtained by means of sequence of differential adhesion. The EPCs can form tubes and invade the malignant cell cluster. Key words: Carcinoma,hepatocellular; Endothelial progenitor cell; Endothelial cell; Tube formation; Invasion
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call